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Addgene

pCEV-G3-Km
(Plasmid #46817)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46817 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEV-G2-Km
  • Modifications to backbone
    PGK1 promoter replaced with GAL10 promoter.
  • Vector type
    Yeast Expression
  • Promoter TEF1 and GAL10
  • Selectable markers
    G418
  • Tag / Fusion Protein
    • FLAG and c-myc

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Selection in E. coli can be performed on Amp or Kan. Selection in yeast can be performed on G418. See publication for details
  • Copy number
    High Copy

Cloning Information

  • 5′ sequencing primer see publication and plasmid map for details
  • 3′ sequencing primer see publication and plasmid map for details
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEV-G3-Km was a gift from Lars Nielsen & Claudia Vickers (Addgene plasmid # 46817 ; http://n2t.net/addgene:46817 ; RRID:Addgene_46817)

  • For your References section:

    Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae. Vickers CE, Bydder SF, Zhou Y, Nielsen LK. Microb Cell Fact. 2013 Oct 25;12(1):96. 10.1186/1475-2859-12-96 PubMed 24161108
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