pCEV-G2-Km
(Plasmid #46815)

• Purpose: (Empty Backbone) TEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformation
• Depositing Labs: Lars Nielsen, Claudia Vickers
• Publication: Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Backbone

• Vector backbone: pSP-G2
• Modifications to backbone: URA3 gene replaced with aphA1 gene for G418/kanamycin/neomycin resistance
• Vector type: Yeast Expression
• Promoter: TEF1 and PGK1
• Selectable markers: G418
• Tag / Fusion Protein:
  • FLAG and c-myc

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Selection in E. coli can be performed on Amp or Kan. Selection in yeast can be performed on G418. See publication for details
• Copy number: High Copy

Cloning Information

• 5′ sequencing primer: see publication for details
• 3′ sequencing primer: see publication for details

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry