pHAGE PGK-GFP-IRES-LUC-W
(Plasmid #46793)

• Purpose: 2nd generation lentiviral vector
• Depositing Lab: Darrell Kotton
• Publication: Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Backbone

• Vector backbone: pHAGE
• Backbone manufacturer: Richard Mulligan
• Backbone size w/o insert (bp): 4874
• Total vector size (bp): 9272
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: PGK
• Alt name: PGK promoter
• Species: H. sapiens (human)
• Insert Size (bp): 465

Gene/Insert 2

• Gene/Insert name: eGFP
• Insert Size (bp): 720
• Entrez Gene: eGFP (a.k.a. pPRS3a_01)

Gene/Insert 3

• Gene/Insert name: IRES
• Alt name: Internal ribosomal entry site
• Insert Size (bp): 578

Gene/Insert 4

• Gene/Insert name: Luciferase
• Insert Size (bp): 1653

Resource Information

• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry