Rab8-T22N(Flag) in pcDNA3.1neo
(Plasmid #46784)

• Purpose: expression of T22N Rab8a in mammalian cells
• Depositing Lab: Terry Hébert
• Publication: Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7.

Backbone

• Vector backbone: pcDNA3.1neo
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RAB8A T22N
• Alt name: RAB8
• Alt name: member RAS oncogene family
• Alt name: RAB8A
• Species: H. sapiens (human)
• Insert Size (bp): 610
• Mutation: T22N dominant negative
• Entrez Gene: RAB8A (a.k.a. MEL, RAB8)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • Rab8 T22N sequence data
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments