PCMV-intron myc Rab1aWT
(Plasmid #46776)

• Purpose: expression of WT Rab1a in mammalian cells
• Depositing Lab: Terry Hébert
• Publication: Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7.

Backbone

• Vector backbone: pCMV-intron myc
• Modifications to backbone: intron-myc inserted HindIII/XbaI. Intron enhances stability of mRNA.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rab1a
• Alt name: member RAS oncogene family
• Species: H. sapiens (human)
• Entrez Gene: RAB1A (a.k.a. RAB1, YPT1)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pCMV-intron-myc-sequence
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cDNA was obtained from UMR cDNA Resource Center, www.cdna.org .

Also see Dupré et al., 2006 Cell Signal. 2007 Mar;19(3):481-9. https://www.ncbi.nlm.nih.gov/pubmed/16979872 for more information.