pKIKOlacZKm
(Plasmid #46767)

• Purpose: (Empty Backbone) E.coli genomic integration at lacZ
• Depositing Labs: Lars Nielsen, Claudia Vickers
• Publication: Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Backbone

• Vector backbone: pKD4
• Backbone size (bp): 3267
• Modifications to backbone: oriR6Kgamma bla fragment from pKD4 used as backbone. 1kb fragment of lacZ added (creating an intermediate vector). Subsequent addition of MCS and kanamycin antibiotic resistance cassette
• Vector type: E.coli genomic integration

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Low Copy

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is used for integration of DNA into the E.coli genome via homologous recombination. The DNA of interest is cloned between lacZ homologous arms and integrated at the lacZ locus.