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Addgene

pKIKOarsBKm
(Plasmid #46766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKD4
  • Backbone size (bp) 3267
  • Modifications to backbone
    oriR6Kgamma bla fragment from pKD4 used as backbone. 1kb fragment of arsB added (creating an intermediate vector). Subsequent addition of MCS and kanamycin antibiotic resistance cassette
  • Vector type
    E.coli genomic integration

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    Low Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is used for integration of DNA into the E.coli genome via homologous recombination. The DNA of interest is cloned between arsB homologous arms and integrated at the arsB locus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKIKOarsBKm was a gift from Lars Nielsen & Claudia Vickers (Addgene plasmid # 46766 ; http://n2t.net/addgene:46766 ; RRID:Addgene_46766)

  • For your References section:

    Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci. Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE. Microb Cell Fact. 2013 Jun 24;12(1):60. 10.1186/1475-2859-12-60 PubMed 23799955
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