pKIKOarsBCm
(Plasmid #46763)

• Purpose: (Empty Backbone) E.coli genomic integration at arsB
• Depositing Labs: Lars Nielsen, Claudia Vickers
• Publication: Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Backbone

• Vector backbone: pKD4
• Backbone size (bp): 3267
• Modifications to backbone: oriR6Kgamma bla fragment from pKD4 used as backbone. 1kb fragment of arsB added (creating an intermediate vector). Subsequent addition of MCS and chloramphenicol antibiotic resistance cassette.
• Vector type: E.coli genomic integration

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Low Copy

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is used for integration of DNA into the E.coli genome via homologous recombination. The DNA of interest is cloned between arsB homologous arms and integrated at the arsB locus.

This plasmid has been found exist in our stock as a multimer. Plasmid multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.