pT3TS-nCas9n Notes
Addgene Diagnostic Digest
This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid pT3TS-nCas9n with the following enzymes: AgeI-HF and HindIII-HF.
Expected Bands
AgeI-HF: 7.3 kb
HindIII-HF: 7.3 kb
AgeI-HF/ HindIII-HF: 4.2 kb & 3.2 kb
We observe the expected bands for this digest.