pT3TS-nCas9n
(Plasmid #46757)

• Depositing Lab: Wenbiao Chen
• Publication: Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Backbone

• Vector backbone: pT3T7
• Backbone size w/o insert (bp): 2800
• Total vector size (bp): 7330
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9
• Alt name: csn1
• Species: Synthetic
• Insert Size (bp): 4400
• Promoter: T3
• Tags / Fusion Proteins:
  • SV40 NLS (N terminal on insert)
  • SV40 NLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: M13 R
• 3′ sequencing primer: M13 F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 99 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information on Chen and Wente Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Chen/