pQHA-USP7 CS puroR
(Plasmid #46754)

• Purpose: Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7
• Depositing Labs: Goedele Maertens, Gordon Peters
• Publication: Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub 2010 Jul 2.

Backbone

• Vector backbone: pQCXIP
• Backbone manufacturer: Clontech
• Total vector size (bp): 10488
• Modifications to backbone: pQHA-puro was created by ligation into the MfeI- and AgeI-digested pQFlag-puro (pQFlag-puro was engineered by ligation in NotI- and BamHI-digested pQCXIP, giving the following MCS: NotI-Kozak-MfeI-Flag-AgeI-XhoI-BamHI).
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: USP7
• Species: H. sapiens (human)
• Mutation: C223S--catalytically inactive
• Entrez Gene: USP7 (a.k.a. C16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: pCDH-Rev

Resource Information

• Supplemental Documents:
  • pQHA-USP7 CS puro
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Sequence discrepancies between the full and QC sequences have no functional consequence.