pBABE-Puro-KRas*G12V
(Plasmid #46746)

• Purpose: Expresses human oncogenic KRas protein from chimeric cDNA sequence derived primarily from HRAS codons with a KRAS 3' tail. Results in higher than normal exogenous expression of KRas protein.
• Depositing Lab: Christopher Counter
• Publication: Lampson et al Curr Biol. 2013 Jan 7;23(1):70-5. doi: 10.1016/j.cub.2012.11.031. Epub 2012 Dec 13.

Backbone

• Vector backbone: pBABE-Puro
• Backbone size w/o insert (bp): 5169
• Total vector size (bp): 5741
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KRAS*G12V
• Alt name: V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 591
• Mutation: carries the oncogenic G12V mutation and cDNA is encoded by a chimeric mixture of HRAS and KRAS codons that encode KRas protein
• Entrez Gene: KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry