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Addgene

pcDNA3.2 V5-DEST 3XFlag-SRSF3
(Plasmid #46736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.2/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 8038
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRSF3
  • Alt name
    SRp20
  • Species
    H. sapiens (human)
  • Entrez Gene
    SRSF3 (a.k.a. SFRS3, SRp20)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x Flag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.2 V5-DEST 3XFlag-SRSF3 was a gift from David Bartel (Addgene plasmid # 46736 ; http://n2t.net/addgene:46736 ; RRID:Addgene_46736)

  • For your References section:

    Beyond Secondary Structure: Primary-Sequence Determinants License Pri-miRNA Hairpins for Processing. Auyeung VC, Ulitsky I, McGeary SE, Bartel DP. Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031. 10.1016/j.cell.2013.01.031 PubMed 23415231
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