pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1
(Plasmid #46676)

• Depositing Lab: David Bartel
• Publication: Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.

Backbone

• Vector backbone: pcDNA3.2 V5-DEST mir-1-1 reporter
• Backbone manufacturer: Bartel lab (Addgene plasmid# 46646)
• Backbone size w/o insert (bp): 8038
• Vector type: Mammalian Expression, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hsa-mir-133a-1
• Alt name: mir-133a-1 pri-mRNA
• Species: H. sapiens (human)
• Entrez Gene: MIR133A1 (a.k.a. MIRN133A1, mir-133a-1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • hsa-mir-1-1 (C terminal on insert)
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: TK-pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry