pcDNA3.2 mir-1-1 reporter cel-lin-4
(Plasmid
#46649)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46649 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.2 V5-DEST mir-1-1 reporter
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Backbone manufacturerBartel lab (Addgene plasmid# 46646)
- Backbone size w/o insert (bp) 8038
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Vector typeMammalian Expression, RNAi
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecel-lin-4
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Alt namelin-4 pri-mRNA
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SpeciesC. elegans (nematode)
- Promoter CMV
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Tags
/ Fusion Proteins
- hsa-mir-1-1 (C terminal on insert)
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer TK-pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.2 mir-1-1 reporter cel-lin-4 was a gift from David Bartel (Addgene plasmid # 46649 ; http://n2t.net/addgene:46649 ; RRID:Addgene_46649) -
For your References section:
Beyond Secondary Structure: Primary-Sequence Determinants License Pri-miRNA Hairpins for Processing. Auyeung VC, Ulitsky I, McGeary SE, Bartel DP. Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031. 10.1016/j.cell.2013.01.031 PubMed 23415231