pCOCK CMVwt
(Plasmid #46559)

• Purpose: Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.
• Depositing Lab: Clifford Wang
• Publication: Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 10.1007/s11693-011-9089-0. Epub 2011 Nov 20.

Backbone

• Vector backbone: pEF5-FRT-V5-DEST
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 7528
• Total vector size (bp): 6184
• Modifications to backbone: 1. EF1alpha promoter removed (so attR Gateway sites can be used to clone in synthetic promoters) 2. IVS-EGFP cassette inserted between attR Gateway cassette and BGH polyA 3. Synthetic promoters flanked by attL in pDONR221 (Life Technologies) vectors were used to recombine promoters into the vector resulting from modifications 1 and 2 above.
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Hygromycin ; No ATG until recombines into FRT site in target cell genome

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CMV wt promoter
• Species: Cytomegalovirus viral promoter
• Insert Size (bp): 589
• Mutation: Our version has a G at base 724 (reference has C)
• GenBank ID: # K03104.1 (bases 156 to 744)
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV 5' end primer
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • pCOCK CMVwt full DNA sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry