Skip to main content
Addgene

pGEX Nck2-SH2
(Plasmid #46458)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46458 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX6P1
  • Backbone manufacturer
    GE Healthcare Biosciences
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NM_001004720.2
  • Alt name
    Nck2
  • Alt name
    GRB4; NCKbeta; NCK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    334
  • Mutation
    contains amino acids 274-380
  • Entrez Gene
    NCK2 (a.k.a. GRB4, NCKbeta)
  • Promoter Tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • PreScission (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The molecular weight of the GST fusion protein is approximately 37.1 kDa

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX Nck2-SH2 was a gift from Bruce Mayer (Addgene plasmid # 46458 ; http://n2t.net/addgene:46458 ; RRID:Addgene_46458)

  • For your References section:

    High-throughput phosphotyrosine profiling using SH2 domains. Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, Saksela K, Schiller MR, Shin DG, Mayer BJ. Mol Cell. 2007 Jun 22;26(6):899-915. 10.1016/j.molcel.2007.05.031 PubMed 17588523
Related items:
Commonly requested with: