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pGEX Grb2-SH2 RK
(Plasmid #46441)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46441 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX6P1
  • Backbone manufacturer
    GE Healthcare Biosciences
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Grb2 RK
  • Alt name
    GRB2
  • Alt name
    ASH; Grb3-3; MST084; MSTP084; EGFRBP-GRB2; GRB2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    318
  • Mutation
    contains amino acids 58-159
  • Entrez Gene
    GRB2 (a.k.a. ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2)
  • Promoter Tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • PreScission (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The molecular weight of the GST fusion protein is approximately 36.6 kDa

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX Grb2-SH2 RK was a gift from Bruce Mayer (Addgene plasmid # 46441 ; http://n2t.net/addgene:46441 ; RRID:Addgene_46441)

  • For your References section:

    High-throughput phosphotyrosine profiling using SH2 domains. Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, Saksela K, Schiller MR, Shin DG, Mayer BJ. Mol Cell. 2007 Jun 22;26(6):899-915. 10.1016/j.molcel.2007.05.031 PubMed 17588523
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