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pGEX RASGAP(NC)-SH2
(Plasmid #46433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46433 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX2T
  • Backbone manufacturer
    GE Healthcare Biosciences
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Gap/RASGAP(NC)
  • Alt name
    RASA1
  • Alt name
    GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP; DKFZp434N071; RASA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    854
  • Mutation
    contains amino acids 171-448
  • Entrez Gene
    RASA1 (a.k.a. CM-AVM, CMAVM, CMAVM1, GAP, PKWS, RASA, RASGAP, p120, p120GAP, p120RASGAP)
  • Promoter Tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The molecular weight of the GST fusion protein is approximately 54.5 kDa

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX RASGAP(NC)-SH2 was a gift from Bruce Mayer (Addgene plasmid # 46433 ; http://n2t.net/addgene:46433 ; RRID:Addgene_46433)

  • For your References section:

    High-throughput phosphotyrosine profiling using SH2 domains. Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, Saksela K, Schiller MR, Shin DG, Mayer BJ. Mol Cell. 2007 Jun 22;26(6):899-915. 10.1016/j.molcel.2007.05.031 PubMed 17588523
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