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Addgene

pAC4 Frk-SH2
(Plasmid #46428)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAC4
  • Backbone manufacturer
    Avidity
  • Backbone size w/o insert (bp) 4216
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Frk
  • Alt name
    FRK
  • Alt name
    GTK; RAK; PTK5; FRK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    332
  • Mutation
    contains amino acids 108-213
  • Entrez Gene
    FRK (a.k.a. GTK, PTK5, RAK)
  • Promoter Trc
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • Thrombin site (N terminal on backbone)
    • AviTag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pBAD-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The molecular weight of the GST fusion protein is approximately 37.1 kDa

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC4 Frk-SH2 was a gift from Bruce Mayer (Addgene plasmid # 46428 ; http://n2t.net/addgene:46428 ; RRID:Addgene_46428)

  • For your References section:

    High-throughput phosphotyrosine profiling using SH2 domains. Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, Saksela K, Schiller MR, Shin DG, Mayer BJ. Mol Cell. 2007 Jun 22;26(6):899-915. 10.1016/j.molcel.2007.05.031 PubMed 17588523
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