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pcDNA3.1 EWS-myc-HIS
(Plasmid #46386)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46386 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)B/myc-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EWSR1
  • Alt name
    EWS
  • Alt name
    EWS RNA-binding protein 1 isoform CRA_e
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2600
  • Entrez Gene
    EWSR1 (a.k.a. EWS, EWS-FLI1, bK984G1.4)
  • Promoter CMV
  • Tags / Fusion Proteins
    • myc (C terminal on backbone)
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EWS cDNA was amplified by PCR using XhoI-NotI-forward and EcoRI-reverse primers 5-TCTCTCGAGCG
GCCGCGCCACCATGGCGTCCACGGATTACAGTACC-3
5-CCGAATTCGTAGGGCCGATCTCTGCGCTCCTG-3, respectively.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1 EWS-myc-HIS was a gift from Heinz Gehring (Addgene plasmid # 46386 ; http://n2t.net/addgene:46386 ; RRID:Addgene_46386)

  • For your References section:

    Identification of proteins interacting with protein arginine methyltransferase 8: the Ewing sarcoma (EWS) protein binds independent of its methylation state. Pahlich S, Zakaryan RP, Gehring H. Proteins. 2008 Sep;72(4):1125-37. doi: 10.1002/prot.22004. 10.1002/prot.22004 PubMed 18320585
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