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Addgene

pJDJRSF05
(Plasmid #46379)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46379 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3559
  • Total vector size (bp) 4309
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NpuDnaE intein inactive
  • Alt name
    inactive intein with N-terminal H6-Smt3 fusion tag
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    750
  • Mutation
    C1A
  • Promoter T7
  • Tag / Fusion Protein
    • H6-Smt3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJDJRSF05 was a gift from Hideo Iwai (Addgene plasmid # 46379 ; http://n2t.net/addgene:46379 ; RRID:Addgene_46379)

  • For your References section:

    NMR resonance assignment of DnaE intein from Nostoc punctiforme. Heinamaki K, Oeemig JS, Paakkonen K, Djupsjobacka J, Iwai H. Biomol NMR Assign. 2009 Jun;3(1):41-3. doi: 10.1007/s12104-008-9137-1. Epub 2009 Jan 13. 10.1007/s12104-008-9137-1 PubMed 19636943
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