Skip to main content
Addgene

pHYRSF236
(Plasmid #46377)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pRSF-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3559
  • Total vector size (bp) 4300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NpuDnaE intein delta variant, inactive
  • Alt name
    cis-splicing minimized intein with N-terminal H6-Smt3 fusion tag
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    741
  • Mutation
    C1A, C+1A, deleted T76, V97, D98, L100 from NpuDnaE intein
  • Promoter T7
  • Tag / Fusion Protein
    • H6-Smt3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHYRSF236 was a gift from Hideo Iwai (Addgene plasmid # 46377 ; http://n2t.net/addgene:46377 ; RRID:Addgene_46377)

  • For your References section:

    Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. 10.1038/nchembio.1320 PubMed 23974115
Related items:
Commonly requested with: