pHYRSF236
(Plasmid
#46377)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSF-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3559
- Total vector size (bp) 4300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNpuDnaE intein delta variant, inactive
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Alt namecis-splicing minimized intein with N-terminal H6-Smt3 fusion tag
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SpeciesNostoc punctiforme
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Insert Size (bp)741
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MutationC1A, C+1A, deleted T76, V97, D98, L100 from NpuDnaE intein
- Promoter T7
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Tag
/ Fusion Protein
- H6-Smt3 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHYRSF236 was a gift from Hideo Iwai (Addgene plasmid # 46377 ; http://n2t.net/addgene:46377 ; RRID:Addgene_46377) -
For your References section:
Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. 10.1038/nchembio.1320 PubMed 23974115