pSABAD518
(Plasmid
#46374)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46374 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5097
- Total vector size (bp) 5686
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTvoVMA intein, inactive
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Alt nameinactive cis-splicing intein with H-GB1 and GB1 as N-extein and SH3 as C-exteins
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SpeciesThermoplasma volcanium
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Insert Size (bp)1089
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MutationN186A, T+1A mutations, inactive
- Promoter pBAD
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Tags
/ Fusion Proteins
- H6-GB1 (N terminal on insert)
- SH3 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSABAD518 was a gift from Hideo Iwai (Addgene plasmid # 46374 ; http://n2t.net/addgene:46374 ; RRID:Addgene_46374) -
For your References section:
Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. 10.1038/nchembio.1320 PubMed 23974115