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Addgene

pSADuet502
(Plasmid #46373)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3564
  • Total vector size (bp) 4419
  • Modifications to backbone
    removed the second MCS of pRSFDuet-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Toxic for E. coli.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NpuDnaB intein
  • Alt name
    cis-splicing intein with H-GB1 and GB1 as N- and C-exteins
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    1704
  • Promoter T7
  • Tags / Fusion Proteins
    • H6-GB1 (N terminal on insert)
    • GB1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSADuet502 was a gift from Hideo Iwai (Addgene plasmid # 46373 ; http://n2t.net/addgene:46373 ; RRID:Addgene_46373)

  • For your References section:

    Intermolecular domain swapping induces intein-mediated protein alternative splicing. Aranko AS, Oeemig JS, Kajander T, Iwai H. Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320. 10.1038/nchembio.1320 PubMed 23974115
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