pGL3-B-pGAS5
(Plasmid #46371)

• Depositing Labs: Emanuela Felley-Bosco, Rolf Stahel
• Publication: Renganathan et al Mol Cancer. 2014 May 23;13:119. doi: 10.1186/1476-4598-13-119.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 4818
• Total vector size (bp): 5700
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GAS5
• Alt name: growth arrest-specific 5 (non-protein coding)
• Species: H. sapiens (human)
• Insert Size (bp): 837
• Mutation: promoter
• GenBank ID: 60674
• Entrez Gene: GAS5 (a.k.a. NCRNA00030, SNHG2)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This promoter from a cancer line expressing high levels of GAS5 and contains two mismatches in the GAS5 region.