pET21-Streptavidin-Dead Aspartate Loop
(Plasmid
#46368)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46368 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET21
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 5800
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDh5alpha for propagation of the plasmid. Bl21[DE3] or similar required for protein expression.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameStreptavidin-Dead Aspartate Loop
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Alt nameDd
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SpeciesE. coli
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Insert Size (bp)423
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21-Streptavidin-Dead Aspartate Loop was a gift from Mark Howarth (Addgene plasmid # 46368 ; http://n2t.net/addgene:46368 ; RRID:Addgene_46368) -
For your References section:
Plug-And-Play Pairing Via Defined Divalent Streptavidins. Fairhead M, Krndija D, Lowe ED, Howarth M. J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016. 10.1016/j.jmb.2013.09.016 PubMed 24056174