pET21-Streptavidin-Glutamate_Tag
(Plasmid #46367)

• Depositing Lab: Mark Howarth
• Publication: Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 10.1016/j.jmb.2013.09.016.

Backbone

• Vector backbone: pET21
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 5800
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Dh5alpha for propagation of plasmid. Bl21[DE3] or similar for protein expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Streptavidin-Glutamate_Tag
• Alt name: SAe
• Species: E. coli
• Insert Size (bp): 408
• Promoter: T7
• Tag / Fusion Protein:
  • 6 glutamate tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry