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pcDNA3-ClopHensor-H148G-V224L
(Plasmid #46366)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5363
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ClopHensor-H148G-V224L
  • Alt name
    E2GFP-H148G-V224L fused to DsRed-monomer
  • Insert Size (bp)
    1485
  • Mutation
    E2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L
  • Tag / Fusion Protein
    • Strep-II (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-ClopHensor-H148G-V224L was a gift from Daniele Arosio (Addgene plasmid # 46366 ; http://n2t.net/addgene:46366 ; RRID:Addgene_46366)

  • For your References section:

    Calibration and functional analysis of three genetically encoded Cl(-)/pH sensors. Mukhtarov M, Liguori L, Waseem T, Rocca F, Buldakova S, Arosio D, Bregestovski P. Front Mol Neurosci. 2013 Apr 18;6:9. doi: 10.3389/fnmol.2013.00009. Print 2013. 10.3389/fnmol.2013.00009 PubMed 23616745
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