pSCT-GAL93-DEST
(Plasmid
#46339)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46339 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSCT-GAL33VP80
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Backbone manufacturergift of W. Schaffner, University of Zurich
- Backbone size (bp) 6922
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Modifications to backboneMade by cutting out recombination sites from pEF-DEST51 (Invitrogen) with BclI and inserting fragment into pSCT-GALVP80 cut with BamHI/PvuII.
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Vector typeMammalian Expression
- Promoter pCMV
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Tag
/ Fusion Protein
- 6XHIS (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSCT-GAL93-DEST was a gift from Steven Brown (Addgene plasmid # 46339 ; http://n2t.net/addgene:46339 ; RRID:Addgene_46339) -
For your References section:
Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, Kramer A, Brown SA. Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. Epub 2012 Sep 10. 10.1128/MCB.00334-12 PubMed 22966205
Map uploaded by the depositor.
