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Addgene

pSCT-GAL93-DEST
(Plasmid #46339)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSCT-GAL33VP80
  • Backbone manufacturer
    gift of W. Schaffner, University of Zurich
  • Backbone size (bp) 6922
  • Modifications to backbone
    Made by cutting out recombination sites from pEF-DEST51 (Invitrogen) with BclI and inserting fragment into pSCT-GALVP80 cut with BamHI/PvuII.
  • Vector type
    Mammalian Expression
  • Promoter pCMV
  • Tag / Fusion Protein
    • 6XHIS (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Unknown

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSCT-GAL93-DEST was a gift from Steven Brown (Addgene plasmid # 46339 ; http://n2t.net/addgene:46339 ; RRID:Addgene_46339)

  • For your References section:

    Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, Kramer A, Brown SA. Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. Epub 2012 Sep 10. 10.1128/MCB.00334-12 PubMed 22966205
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