Skip to main content
Addgene

pG4-E6s-luc
(Plasmid #46324)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46324 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGL3-E6s
  • Backbone manufacturer
    Brown lab
  • Total vector size (bp) 5243
  • Modifications to backbone
    E boxes from Dpb inserted upstream of Luc reporter in pGL3.
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5xGal4
  • Alt name
    Gal4
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    122
  • Mutation
    five copies of a multimerized GAL4 (5×GAL4) site (cut from pFR-luc; Invitrogen)
  • Tags / Fusion Proteins
    • E boxes (C terminal on backbone)
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pG4-E6s-luc was a gift from Steven Brown (Addgene plasmid # 46324 ; http://n2t.net/addgene:46324 ; RRID:Addgene_46324)

  • For your References section:

    Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, Kramer A, Brown SA. Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. Epub 2012 Sep 10. 10.1128/MCB.00334-12 PubMed 22966205
Related items:
Commonly requested with: