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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46320 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSCT-GAL93-DEST
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Backbone manufacturerBrown lab (Addgene plasmid 46339)
- Total vector size (bp) 7409
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSFPQ
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Alt namesplicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)
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Alt namePSF
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Alt nameREP1
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SpeciesH. sapiens (human)
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GenBank IDNM_005066.2
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Entrez GeneSFPQ (a.k.a. POMP100, PPP1R140, PSF)
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a stop codon before HIS tag in the vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSCT-GAL93-SFPQ was a gift from Steven Brown (Addgene plasmid # 46320 ; http://n2t.net/addgene:46320 ; RRID:Addgene_46320) -
For your References section:
Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, Kramer A, Brown SA. Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. Epub 2012 Sep 10. 10.1128/MCB.00334-12 PubMed 22966205
Map uploaded by the depositor.