Hela kinase-dead MLCK-GFP
(Plasmid #46317)

• Depositing Lab: Anne Bresnick
• Publication: Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-93.

Backbone

• Vector backbone: pEGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 10500
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HeLa long myosin light chain kinase-dead
• Alt name: human full-length MLCK 210 kinase-dead
• Species: H. sapiens (human)
• Insert Size (bp): 5800
• Mutation: E1626K
• GenBank ID: NC_000003.11
• Entrez Gene: MYLK (a.k.a. AAT7, KRP, MLCK, MLCK1, MLCK108, MLCK210, MMIHS, MMIHS1, MSTP083, MYLK-L, MYLK1, smMLCK)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry