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Addgene

HA-Vim
(Plasmid #46315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-HA
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vimentin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1416
  • Entrez Gene
    Vim
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EBV-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-Vim was a gift from Xiangli Yang (Addgene plasmid # 46315 ; http://n2t.net/addgene:46315 ; RRID:Addgene_46315)

  • For your References section:

    Vimentin inhibits ATF4-mediated osteocalcin transcription and osteoblast differentiation. Lian N, Wang W, Li L, Elefteriou F, Yang X. J Biol Chem. 2009 Oct 30;284(44):30518-25. Epub 2009 Sep 2. 10.1074/jbc.M109.052373 PubMed 19726676
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