SI11-Sema4c
(Plasmid #46301)

• Depositing Lab: Gavin Wright
• Publication: Crosnier et al BMC Biol. 2010 . 8():76.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 5925
• Total vector size (bp): 8350
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sema4c mouse monoclonal antibody
• Alt name: sc:d411
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2400
• Entrez Gene: sc:d411 (a.k.a. CH211-244O22.3)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: M13 rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada - Biotechnology Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results contain a few nucleotide mismatches when compared to the sequence provided by the depositing laboratory. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.