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Addgene

SI11-Sema4c
(Plasmid #46301)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46301 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 5925
  • Total vector size (bp) 8350
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sema4c mouse monoclonal antibody
  • Alt name
    sc:d411
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2400
  • Entrez Gene
    sc:d411 (a.k.a. CH211-244O22.3)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13 rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada - Biotechnology Research Institute

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results contain a few nucleotide mismatches when compared to the sequence provided by the depositing laboratory. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SI11-Sema4c was a gift from Gavin Wright (Addgene plasmid # 46301 ; http://n2t.net/addgene:46301 ; RRID:Addgene_46301)
  • For your References section:

    A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. Crosnier C, Staudt N, Wright GJ. BMC Biol. 2010 . 8():76. 10.1186/1741-7007-8-76 PubMed 20525357