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Addgene

pGW_PSD95.FingR-YFP
(Plasmid #46299)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46299 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGW_YFP
  • Backbone size w/o insert (bp) 5541
  • Total vector size (bp) 5860
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PSD95.FingR
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    318
  • Entrez Gene
    DLG4 (a.k.a. MRD62, PSD95, SAP-90, SAP90)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CTTAGGCACAGCACG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGW_PSD95.FingR-YFP was a gift from Don Arnold (Addgene plasmid # 46299 ; http://n2t.net/addgene:46299 ; RRID:Addgene_46299)
  • For your References section:

    Recombinant probes for visualizing endogenous synaptic proteins in living neurons. Gross GG, Junge JA, Mora RJ, Kwon HB, Olson CA, Takahashi TT, Liman ER, Ellis-Davies GC, McGee AW, Sabatini BL, Roberts RW, Arnold DB. Neuron. 2013 Jun 19;78(6):971-85. doi: 10.1016/j.neuron.2013.04.017. 10.1016/j.neuron.2013.04.017 PubMed 23791193