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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46298 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGW_YFP
- Backbone size w/o insert (bp) 5541
- Total vector size (bp) 5860
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGPHN.FingR
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)318
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Entrez GeneGPHN (a.k.a. GEPH, GPH, GPHRYN, HKPX1, MOCODC)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CTTAGGCACAGCACG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGW_GPHN.FingR-YFP was a gift from Don Arnold (Addgene plasmid # 46298 ; http://n2t.net/addgene:46298 ; RRID:Addgene_46298) -
For your References section:
Recombinant probes for visualizing endogenous synaptic proteins in living neurons. Gross GG, Junge JA, Mora RJ, Kwon HB, Olson CA, Takahashi TT, Liman ER, Ellis-Davies GC, McGee AW, Sabatini BL, Roberts RW, Arnold DB. Neuron. 2013 Jun 19;78(6):971-85. doi: 10.1016/j.neuron.2013.04.017. 10.1016/j.neuron.2013.04.017 PubMed 23791193