pCAG_GPHN.FingR-mKate2-IL2RGTC
(Plasmid #46297)

• Depositing Lab: Don Arnold
• Publication: Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 10.1016/j.neuron.2013.04.017.

Backbone

• Vector backbone: pCAG
• Backbone size w/o insert (bp): 5873
• Total vector size (bp): 7415
• Modifications to backbone: DNA binding site to IL2RG left-handed zinc finger (CTTCCACAGAGT) inserted upstream of the CAG promoter.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GPHN.FingR-mKate2-IL2RGTC
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1542
• Entrez Gene: GPHN (a.k.a. GEPH, GPH, GPHRYN, HKPX1, MOCODC)
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: BsrGI (destroyed during cloning)
• 5′ sequencing primer: TCCTACAGCTCCTGG
• 3′ sequencing primer: CCCAGTTTCTATGTGGTCT

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry