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pCAG_GPHN.FingR-eGFP-CCR5TC
(Plasmid #46296)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46296 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 4113
  • Total vector size (bp) 7483
  • Modifications to backbone
    DNA binding site to CCR5 left-handed zinc finger (GTCATCCTCATC) inserted upstream of the CAG promoter.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GPHN.FingR-eGFP-CCR5TC
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1599
  • Entrez Gene
    GPHN (a.k.a. GEPH, GPH, GPHRYN, HKPX1, MOCODC)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer TCCTACAGCTCCTGG
  • 3′ sequencing primer CCCAGTTTCTATGTGGTCT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG_GPHN.FingR-eGFP-CCR5TC was a gift from Don Arnold (Addgene plasmid # 46296 ; http://n2t.net/addgene:46296 ; RRID:Addgene_46296)

  • For your References section:

    Recombinant probes for visualizing endogenous synaptic proteins in living neurons. Gross GG, Junge JA, Mora RJ, Kwon HB, Olson CA, Takahashi TT, Liman ER, Ellis-Davies GC, McGee AW, Sabatini BL, Roberts RW, Arnold DB. Neuron. 2013 Jun 19;78(6):971-85. doi: 10.1016/j.neuron.2013.04.017. 10.1016/j.neuron.2013.04.017 PubMed 23791193
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