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pmECFPN3/GgVcl 1-1066 (CFP at C term)
(Plasmid #46280)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46280 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-N3
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7924
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vcl 1-1066
  • Alt name
    Vinculin
  • Alt name
    Vcl
  • Species
    G. gallus (chicken)
  • Entrez Gene
    VCL (a.k.a. VINC1)
  • Promoter CMV
  • Tag / Fusion Protein
    • ECFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A206K was introduced into pECFP-N3/V1-1066 by QC to generate pmECFP/V1066 (vinculin-monomeric ECFP)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmECFPN3/GgVcl 1-1066 (CFP at C term) was a gift from Susan Craig (Addgene plasmid # 46280 ; http://n2t.net/addgene:46280 ; RRID:Addgene_46280)

  • For your References section:

    Spatial distribution and functional significance of activated vinculin in living cells. Chen H, Cohen DM, Choudhury DM, Kioka N, Craig SW. J Cell Biol. 2005 May 9;169(3):459-70. 10.1083/jcb.200410100 PubMed 15883197
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