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Addgene

mcherry(C1)_RepoMan/RAXA
(Plasmid #46275)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46275 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmcherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7772
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RepoMan
  • Alt name
    CDCA2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3072
  • Mutation
    V393A/F395A
  • GenBank ID
    NM_152562
  • Entrez Gene
    CDCA2 (a.k.a. PPP1R81, Repo-Man)
  • Tag / Fusion Protein
    • mcherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Asp718 (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer CAACGTCAACATCAAGTTGG
  • 3′ sequencing primer GTTTGGACAAACCACAACTAGAATGCAG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mcherry(C1)_RepoMan/RAXA was a gift from Angus Lamond & Laura Trinkle-Mulcahy (Addgene plasmid # 46275 ; http://n2t.net/addgene:46275 ; RRID:Addgene_46275)

  • For your References section:

    Repo-Man recruits PP1 gamma to chromatin and is essential for cell viability. Trinkle-Mulcahy L, Andersen J, Lam YW, Moorhead G, Mann M, Lamond AI. J Cell Biol. 2006 Feb 27;172(5):679-92. Epub 2006 Feb 21. 10.1083/jcb.200508154 PubMed 16492807
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