pEGFPC3/GgVcl 884-1066 (Vt)
(Plasmid
#46272)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46272 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C3
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5332
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVcl 884-1066 (Vt)
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Alt nameVinculin
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Alt nameVcl
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SpeciesG. gallus (chicken)
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Insert Size (bp)550
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Mutation884-1066 (Vt) tail domain
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GenBank IDNM_205441.1
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Entrez GeneVCL (a.k.a. VINC1)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PstI-ApaI fragment of pGEX3TV884-1066 subcloned into Pst-Apa cut pEGFP-C3)EGFP.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPC3/GgVcl 884-1066 (Vt) was a gift from Susan Craig (Addgene plasmid # 46272 ; http://n2t.net/addgene:46272 ; RRID:Addgene_46272)
