pEGFPC1/GgVcl 1-851 A50I mutant
(Plasmid #46269)

• Depositing Lab: Susan Craig
• Publication: Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15. Epub 2006 Apr 10.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 7316
• Modifications to backbone: vector modified to contain unique Nde, Xho sites.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Vcl 1-851 A50I mutation
• Alt name: Vinculin
• Alt name: Vcl
• Species: G. gallus (chicken)
• Insert Size (bp): 2550
• Mutation: 1-851 (Vh) head domain, A50I mutation
• GenBank ID: NM_205441.1
• Entrez Gene: VCL (a.k.a. VINC1)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40 pA R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A50I mutation introduced into EGFP/V1-851 (Addgene plasmid 46268) by QCPCR mutation.