pcDNA3-FLAG-HA-CAD
(Plasmid #46238)

• Purpose: For expression of HA and FLAG-tagged murine CAD in mammalian cells
• Depositing Lab: Brendan Manning
• Publication: Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. doi: 10.1126/science.1228792. Epub 2013 Feb 21.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 12345
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• Alt name: CAD
• Species: M. musculus (mouse)
• Insert Size (bp): 6943
• GenBank ID: NM_023525
• Entrez Gene: Cad (a.k.a. 2410008J01Rik, AU018859, Cpad)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry