R26/P-nlsrtTA
(Plasmid #46183)

• Depositing Lab: Rudolf Jaenisch
• Publication: Wutz et al Nat Genet. 2002 Feb;30(2):167-74. Epub 2002 Jan 7.

Backbone

• Vector backbone: ROSA26-1
• Backbone size w/o insert (bp): 9846
• Total vector size (bp): 11946
• Vector type: Mouse Targeting
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: nlsrtTA
• Insert Size (bp): 1500

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: M13-pUC-Fwd

Gene/Insert 2

• Gene/Insert name: pgk-puro
• Insert Size (bp): 1600

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: nls-rtTA was obtained from Dr. H. Bujard

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry