pET15b/GgVcl 884-1066-ECFP
(Plasmid
#46182)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 46182 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-15b
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Backbone manufacturerEMD
- Backbone size w/o insert (bp) 5700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVcl 884-1066
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Alt nameVinculin
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Alt nameVcl
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SpeciesG. gallus (chicken)
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Mutationaa 884-1066 (tail domain)
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GenBank IDNM_205441.1
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Entrez GeneVCL (a.k.a. VINC1)
- Promoter T7
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- ECFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b/GgVcl 884-1066-ECFP was a gift from Susan Craig (Addgene plasmid # 46182 ; http://n2t.net/addgene:46182 ; RRID:Addgene_46182)