pET15b/ECFP-GgVcl 884-1066 (Vt)
(Plasmid #46180)

• Depositing Lab: Susan Craig
• Publication: Craig lab unpublished plasmids (unpublished)

Backbone

• Vector backbone: pET-15b
• Backbone manufacturer: EMD
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 7013
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Vcl 884-1066
• Alt name: Vinculin
• Alt name: Vcl
• Species: G. gallus (chicken)
• Insert Size (bp): 555
• Mutation: aa 884-1066 (tail domain)
• GenBank ID: NM_205441.1
• Entrez Gene: VCL (a.k.a. VINC1)
• Promoter: T7
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • ECFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CFP subcloned into 15b/V884-1066 (Addgene plasmid 46176) via a PCR strategy which placed Nde sites at the ends of the CFP cDNA and added the spacer region in EGFPC1/V1-1066 at the Cterminus of CFP.