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Addgene

pET15b/EYFP-GgVcl 884-1066-ECFP FRET
(Plasmid #46179)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    EMD
  • Backbone size w/o insert (bp) 5700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vcl 884-1066
  • Alt name
    Vinculin
  • Alt name
    Vcl
  • Species
    G. gallus (chicken)
  • Mutation
    aa 884-1066 (Vt, tail domain)
  • GenBank ID
    NM_205441.1
  • Entrez Gene
    VCL (a.k.a. VINC1)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • EYFP (N terminal on insert)
    • ECFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b/EYFP-GgVcl 884-1066-ECFP FRET was a gift from Susan Craig (Addgene plasmid # 46179 ; http://n2t.net/addgene:46179 ; RRID:Addgene_46179)
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