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Addgene

pET15b/GgVcl 1-258 A50I mutation
(Plasmid #46175)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 46175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    EMD
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 6485
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vcl 1-258 A50I
  • Alt name
    Vinculin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    780
  • Mutation
    aa 1-258 (VD1) A50I mutation; talin binding deficient
  • GenBank ID
    NM_205441.1
  • Entrez Gene
    VCL (a.k.a. VINC1)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Addgene plasmid 46173 for wild-type cloning information. The A50I mutation was introduced by QuikChange mutagenesis (Stratagene).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b/GgVcl 1-258 A50I mutation was a gift from Susan Craig (Addgene plasmid # 46175 ; http://n2t.net/addgene:46175 ; RRID:Addgene_46175)

  • For your References section:

    A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. Cohen DM, Kutscher B, Chen H, Murphy DB, Craig SW. J Biol Chem. 2006 Jun 9;281(23):16006-15. Epub 2006 Apr 10. 10.1074/jbc.M600738200 PubMed 16608855
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